Recent developments in life sciences research: Role of bioinformatics

Life sciences research and development has opened up new challenges and opportunities for bioinformatics. The contribution of bioinformatics advances made possible the mapping of the entire human genome and genomes of many other organisms in just over a decade. These discoveries, along with current efforts to determine gene and protein functions, have improved our ability to understand the root causes of human, animal and plant diseases and find new cures. Furthermore, many future Bioinformatic innovations will likely be spurred by the data and analysis demands of the life sciences. This review briefly describes the role of bioinformatics in biotechnology, drug discovery, biomarkerdiscovery, biological databases, bioinformatic tools, bioinformatic tasks and its application in life sciences research

Development and Validation of LC Method for the Determination of Famciclovir in Pharmaceutical Formulation Using an Experimental Design

A rapid and sensitive RP-HPLC method with UV detection (242 nm) for routine analysis of famciclovir in pharmaceutical formulations was developed. Chromatography was performed with mobile phase containing a mixture of methanol and phosphate buffer (50:50, v/v) with flow rate 1.0 mL min−1. Quantitation was accomplished with internal standard method. The procedure was validated for linearity (correlation coefficient =0.9999), accuracy, robustness and intermediate precision. Experimental design was used for validation of robustness and intermediate precision. To test robustness, three factors were considered; percentage v/v of methanol in mobile phase, flow rate and pH; flow rate, the percentage of organic modifier and pH have considerable important effect on the response. For intermediate precision measure the variables considered were: analyst, equipment and number of days. The RSD value (0.86%, n=24) indicated an acceptable precision of the analytical method. The proposed method was simple, sensitive, precise, accurate and quick and useful for routine quality control

Spectrophotometric Determination of Ziprasidone in Pharmaceutical Formulations

A simple and reproducible spectrophotometric method has been developed for the determination of Ziprasidone hydrochloride monohydrate (ZPS) in bulk and in dosage forms. The method is based on the extraction of the drugs into organic layer of the dye TPooo in presence of 0.1 N hydrochloric acid and the absorbances were measured at 490 nm. Results indicate that the proposed method was simple, sensitive, accurate and reproducible

Fe3O4 nanoparticles mediated synthesis of novel spirooxindole‐dihydropyrimidinone molecules as Hsp90 inhibitors

Heat shock protein 90 (Hsp90) is a validated molecular chaperone considered as the new key recipient for cancer intervention. The current study illustrates the synthesis of novel spirooxindole-dihydropyrimidinones (4a-j) by Fe 3 O 4 nanoparticles intervened synthesis and their Hsp90 ATPase inhibitory activity was investigated by the malachite green assay. All the compounds in the study demonstrated a moderate to potent ATPase inhibitory profile, with IC 50 values ranging from 0.18 to 6.80 μM. Compounds 4j, 4h, 4f, and 4i exhibited maximum inhibitory potential with IC 50 values of 0.18, 0.20, 0.35, and 0.55 μM, respectively. They were found to be better than the standard drug, geldanamycin (Hsp9 ATPase inhibition IC 50 = 0.90 μM). Compounds 4h and 4j with IC 50 values of 22.82 ± 0.532, 20.78 ± 0.234 and 21.32 ± 0.765, 28.43 ± 0.653 µM showed significantly greater potencies against the MCF-7 and HepG2 cell lines, respectively. Compound 4j showed good antioxidant activities in the DPPH test and H 2 O 2 assay (IC 50 = 20.13.23 ± 0.32 and 23.27 ± 0.32 μg/mL) when compared with the standard ascorbic acid (IC 50 = 19.16 ± 0.20 and 20.66 ± 1.09 μg/mL). A molecular docking study was performed to observe the binding efficiency and steric interactions of the lead moiety.Fil: Maddela, Srinubabu. Jawaharlal Nehru Technological University Hyderabad; IndiaFil: Makula, Ajitha. Jawaharlal Nehru Technological University Hyderabad; IndiaFil: Galigniana, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Parambi, Della G. T.. Jouf University; Arabia SauditaFil: Federicci, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Mazaira, Gisela Ileana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Hendawy, Omnia M.. Beni Seuf University; Egipto. Jouf University; Arabia SauditaFil: Dev, Sanal. Al Shifa College of Pharmacy; IndiaFil: Mathew, Githa E.. Grace College Of Pharmacy ; IndiaFil: Mathew, Bijo. Ahalia School of Pharmacy ; Indi